Anxiety / depression / mood
Dysregulated GABAergic/monoaminergic tone and stress physiology.
Decision support, not prescription. You decide.
Candidate agents
6 agents · tap to expand▶InositolLeadEmergingInositol is a well-tolerated glucose isomer with a modest, mostly sub-threshold human signal in depression and PMDD mood symptoms; treat it as a low-risk adjunct with unproven stand-alone efficacy, not a primary antidepressant.expand
Inositol is a well-tolerated glucose isomer with a modest, mostly sub-threshold human signal in depression and PMDD mood symptoms; treat it as a low-risk adjunct with unproven stand-alone efficacy, not a primary antidepressant.
▸Full clinical story
As a precursor of the phosphatidylinositol second-messenger system, inositol is proposed to normalize downstream signaling of serotonergic (5-HT2) and other monoaminergic receptors implicated in mood and panic, offering a rationale distinct from direct receptor agonism.
Emerging but weak. A meta-analysis of double-blind RCTs (n=242 depression, n=70 anxiety) found no statistically significant benefit overall, though inositol produced marginally more responders in depression (p=0.06) and a trend in PMDD (p=0.07), and did not separate from placebo for anxiety/OCD; a separate small crossover RCT (n=20) found inositol at least comparable to fluvoxamine for panic-attack frequency over one month. The 'emerging' grade reflects that human data exist but are small and largely fall short of significance.
Anecdotal community signal only: patients (often in PMDD, anxiety, and 'natural alternative to SSRI' circles) take high-dose myo-inositol powder dissolved in water, frequently stacked with SSRIs or used during PMS luteal windows, and report calmer mood and fewer panic episodes. This is uncontrolled self-report, not evidence of efficacy, and reporting bias is high in supplement forums.
For context only: trials used roughly 12-18 g/day of myo-inositol, typically divided; community use often mirrors this range but tolerability is limited by dose.
Main condition-specific flags are additive serotonergic effect when combined with SSRIs (theoretical, generally mild), possible mood destabilization/switch in bipolar depression, and dose-dependent GI upset (loose stool, nausea) that reduces adherence at effective doses.
A cheap, benign option with a real but modest and largely sub-threshold human signal concentrated in depression and PMDD; reasonable as an adjunct for motivated patients, but clinicians should not expect it to replace established antidepressants and should set modest expectations.
▶PE-22-28LeadPreclinicalPE-22-28 (mini-spadin) is a selective TREK-1 blocker with fast antidepressant-like effects in rodents - but zero human evidence.expand
PE-22-28 (mini-spadin) is a selective TREK-1 blocker with fast antidepressant-like effects in rodents - but zero human evidence.
▸Full clinical story
Blocks the TREK-1 background potassium channel (IC50 ~0.12 nM), a target whose genetic deletion confers a depression-resistant phenotype; downstream it potentiates serotonergic signaling and raises hippocampal CREB phosphorylation. It is a shortened, more stable analog of the natural peptide spadin.
Preclinical only. In mice, PE-22-28 and analogs reduced immobility in the forced-swim test and cut latency-to-eat in novelty-suppressed feeding after a 4-day course, with better TREK-1 affinity and ~23 h in vivo stability vs spadin's ~7 h (PMID 28955242). The concept traces to Mazella et al.'s spadin work (PMID 20405001) and is reviewed in PMID 30291907. There are NO human clinical trials.
Sparse. A LongeCity thread discusses spadin/PE-22-28 as a fast-acting TREK-1 antidepressant and sourcing options, but contains no verified first-hand user experience or self-reported dosing. Reddit is not accessible.
No established or approved human dose exists; all data are rodent parenteral (intraperitoneal) dosing. Do not extrapolate animal doses to humans.
Contraindicated in bipolar disorder (pro-neurogenic, excitability-increasing TREK-1 blockade may destabilize mood cycling or trigger mania) and in cardiac arrhythmia or people taking antiarrhythmic / potassium-channel drugs. No human safety data of any kind. Research use only.
Mechanistically compelling and preclinically promising as a fast antidepressant and neurogenesis stimulant, but PE-22-28 remains an unproven research chemical with no human efficacy or safety data - experimental only, and to be avoided in bipolar or arrhythmia patients.
- Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity ↗
- Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design ↗
- Fighting against depression with TREK-1 blockers: Past and future. A focus on spadin ↗
- Mixed1 corroboratinganecdotal
Informational forum discussion of spadin/PE-22-28 as a fast-acting TREK-1-blocking antidepressant; interest in sourcing the peptide but no verified first-hand user experience reports or self-reported dosing.
No peer data yet. Aggregates appear once ≥3 clinicians log an outcome.
▶OxytocinEmergingIntranasal oxytocin is an investigational add-on for mood/distress with one positive small inpatient RCT whose benefit was concentrated in women; take it as a hypothesis-generating adjunct signal, not established therapy.expand
Intranasal oxytocin is an investigational add-on for mood/distress with one positive small inpatient RCT whose benefit was concentrated in women; take it as a hypothesis-generating adjunct signal, not established therapy.
▸Full clinical story
Oxytocin modulates central stress physiology — dampening HPA-axis/amygdala reactivity and enhancing social-affiliative and therapeutic-alliance processes — which provides a plausible route to reduced depression and general distress rather than a direct monoaminergic mechanism.
Emerging. A double-blind placebo-controlled RCT (N=87 inpatients) of intranasal oxytocin 32 IU twice daily for 4 weeks added to usual care showed greater improvement in depression and general distress but no effect on anxiety or working alliance; a secondary analysis found the benefit was driven almost entirely by female patients. Single small trial with sex-moderated effects — grade honestly remains 'emerging.'
Anecdotal community signal only: oxytocin nasal sprays are used off-label for social anxiety, bonding, low mood, and postpartum contexts, with users reporting transient warmth, reduced social stress, and improved connectedness. These are uncontrolled self-reports; effects are described as short-lived and highly context/expectation dependent, and are not evidence of durable antidepressant action.
For context only: the supporting trial used 32 IU intranasally twice daily for 4 weeks; community intranasal dosing is variable and often lower and as-needed.
Condition-specific flags: benefit appears sex-dependent (weaker or absent in males), effects on anxiety specifically were null, and social/emotional effects can be context-dependent or even negative in some interpersonal settings; caution in pregnancy given uterotonic activity.
A biologically plausible adjunct with a single supportive inpatient RCT and a notable female-predominant response; worth watching and reasonable to consider adjunctively in select cases, but far from a validated stand-alone treatment.
▶N-Acetyl Selank AmidatePreclinicalSelank is a synthetic tuftsin-analog peptide marketed for anxiety on the strength of rodent data only; treat its anxiolytic claim as preclinical and unproven in controlled human trials.expand
Selank is a synthetic tuftsin-analog peptide marketed for anxiety on the strength of rodent data only; treat its anxiolytic claim as preclinical and unproven in controlled human trials.
▸Full clinical story
In animal models Selank shows benzodiazepine-comparable anxiolytic activity and is described as modulating GABAergic tone (alongside effects on enkephalin degradation and monoamine/BDNF systems), fitting the condition's GABAergic lever — but the human relevance of these mechanisms is not established.
Preclinical. The anxiolytic evidence rests on rat models — e.g., Selank reducing anxiety in unpredictable chronic mild stress and augmenting diazepam's effect on the elevated plus maze — with no adequately powered, peer-reviewed double-blind human RCT confirming efficacy. A pharmacology review explicitly notes Selank is poorly studied and sold to consumers as a supplement despite thin data. Grade 'preclinical' is correct.
Anecdotal community signal only: nootropic and biohacker communities use Selank as a subcutaneous/intranasal peptide for acute anxiety, stress, and 'take-the-edge-off' effects without benzodiazepine sedation or dependence, reporting rapid calming that fades over hours. These are uncontrolled self-reports, not evidence of efficacy; product purity and dosing from gray-market sources are unverified.
For context only: community intranasal/subcutaneous use is commonly cited around 250-500 mcg per dose, once or a few times daily in short courses — this is not a validated protocol.
Condition-specific flags: it is a GABAergic agent that may be additive with benzodiazepines, alcohol, and other CNS depressants; it is sold as an unregulated supplement/research chemical with no human safety dataset, and reviews group it with phenibut among under-studied GABAergic agents of concern.
Mechanistically interesting and popular off-label, but the anxiolytic case is animal-only; clinicians should treat human efficacy and long-term safety as unestablished and source/quality as a real risk.
▶SemaxPreclinicalSemax is a synthetic ACTH(4-10) peptide with anxiolytic/antidepressant signals in stressed rodents only; treat its mood claim as preclinical, with no controlled human mood-disorder trials.expand
Semax is a synthetic ACTH(4-10) peptide with anxiolytic/antidepressant signals in stressed rodents only; treat its mood claim as preclinical, with no controlled human mood-disorder trials.
▸Full clinical story
In animals Semax normalizes brain monoamine (serotonin/dopamine/noradrenaline) levels and stress-related behavior and raises BDNF, aligning with the condition's monoaminergic/stress-physiology lever — but notably it acted only under elevated-stress conditions, not in normal-state animals.
Preclinical. Evidence is rodent behavioral: Semax reversed CCK-4-induced anxiety- and depression-like behavior in the elevated plus maze and forced swim test, and attenuated behavioral and monoamine disturbances from neonatal SSRI (fluvoxamine) exposure. There are no peer-reviewed double-blind human RCTs for depression or anxiety; grade 'preclinical' is accurate.
Anecdotal community signal only: nootropic users take intranasal Semax for focus, mood lift, and stress resilience, often stacked with Selank, reporting subtle antidepressant/anti-anxiety and cognitive effects. These are uncontrolled reports on gray-market product, not evidence of efficacy; effects are described as mild and easily confounded with placebo.
For context only: community intranasal use is commonly cited around 250-600 mcg/day (and higher for the N-acetyl 'Semax amidate' variant); no validated mood-disorder dosing exists.
Condition-specific flags: theoretical additive/serotonergic interaction given its monoaminergic effects, unregulated supplement sourcing with no human safety data in mood disorders, and effects that appear stress-condition-dependent, making benefit in euthymic or mildly symptomatic patients uncertain.
A plausible neuromodulatory peptide with encouraging animal mood data but zero controlled human mood evidence; interesting to track, not something to present to patients as a proven antidepressant or anxiolytic.
Not recommended for this condition
Shown to close the loop — the evidence points the wrong way here.
▶SemaglutideEmergingFor mood, semaglutide carries a known-negative safety signal, not a therapeutic one: observational data associate GLP-1 receptor agonist exposure with later antidepressant dispensing, so monitor mood rather than use it to treat it.expand
For mood, semaglutide carries a known-negative safety signal, not a therapeutic one: observational data associate GLP-1 receptor agonist exposure with later antidepressant dispensing, so monitor mood rather than use it to treat it.
▸Full clinical story
The signal is association-level; a mechanistic route through GLP-1 effects on central reward, appetite, and stress circuits is plausible but unproven, and confounding by indication (weight, diabetes, cardiometabolic burden — themselves linked to depression) cannot be excluded in these data.
Emerging safety signal. A large Australian pharmacoepidemiologic study (PBS data) found GLP-1 receptor agonist exposure, including semaglutide, associated with increased subsequent antidepressant dispensing (adjusted HR 1.19; cross-sectional ORs ~1.44-1.52). This is observational, dispensing-based, and cannot establish causation; grade 'emerging' with an 'adverse' direction is the honest characterization.
Anecdotal community signal only: within weight-loss and GLP-1 patient communities there are mixed reports — some describe low mood, anhedonia, or loss of the 'food reward' pleasure, while others report improved mood with weight loss. These conflicting self-reports underscore uncertainty and should not be read as confirming or refuting the pharmacoepidemiologic signal.
Not applicable as a mood therapeutic — there is no antidepressant dosing. In practice semaglutide is dosed for its metabolic indications; mood is a monitoring parameter, not a titration target.
Condition-specific flags: exercise vigilance for new or worsening depression and mood change in patients started on semaglutide/GLP-1 agonists, particularly those with pre-existing psychiatric history; regulators and labels have flagged monitoring for depression and suicidal ideation, so screen at baseline and follow-up.
Treat this as a mood-safety watch item, not a treatment: the evidence is an observational association requiring vigilance, and clinicians should monitor mood in patients on semaglutide rather than expect or induce psychiatric benefit.
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