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Thymosin Beta-4
Healing & RegenerationThymosin Beta-4 is an endogenous peptide critical for cellular repair and cytoskeletal regulation. It plays a key role in tissue regeneration, inflammation control, and angiogenesis. Distinct from synthetic TB-500 fragments, full-length TB4 is being studied for cardiac recovery, neurological healing, and advanced wound care.
Reconstitute
3 mL BAC + 5mg vial
17 mcg/unit
Daily Range
1–4 mg Subcutaneous (SQ)
1–2x weekly
Standard Dose
2 mg
Cycle
4–8 weeks
then reassess
Thymosin Beta-4angiogenesiscytoskeletal repairtissue regenerationcardiac healing
Dosing & Reconstitution Guide
In animal studies, Tβ4 has been administered at 0.5–2.0 mg per dose via subcutaneous or intraperitoneal injection depending on model and study duration. No human dosing guidelines are approved or validated.
Standard / Gradual Approach
standardPhaseDoseVolume
Week 11mg
Week 22mg
Week 33mg
Week 45mg
Protocol Summary
Subcutaneous (SQ): 1–2x weekly · Dose range 1–4 mg with gradual titration
Cycle Length: 4–8 weeks typical; reassess before extending
Frequency & Cycling
SubQ Injection
Loading phase: 2–4 mg SubQ twice weekly for 2–4 weeks. Maintenance phase: 2 mg weekly as needed. Cycles repeated based on injury severity or recovery goals.
🧪 Quick Start
BAC Water
3 mL
Potential Benefits & Use Cases
Thymosin Beta-4 is a research-use-only peptide. It is not approved for human or veterinary use. All information presented is based on preclinical studies and is intended for educational purposes only.
Studied for accelerating wound closure and dermal regeneration in rodents
Investigated for promoting angiogenesis and new blood vessel formation
Explored for cardiac tissue repair following injury in animal models
Evaluated for neuroprotective and anti-inflammatory roles in CNS trauma studies
Supports cell migration and extracellular matrix remodeling in preclinical data
Clinical data Strong preclinical Limited data
Mechanism of Action
→Binds to G-actin, promoting cell migration and cytoskeletal remodeling
→Activates integrin-linked kinase (ILK) for tissue repair signaling
→Increases VEGF expression to stimulate angiogenesis
→Downregulates pro-inflammatory cytokines and chemokines in damaged tissue
Lifestyle & Optimization
timing
Inject SubQ. Loading phase then maintenance.
diet
High protein intake (1.2g per pound body weight). Avoid smoking to support healing.
exercise
Allow adequate recovery between intense workouts (1 rest day per week minimum).
sleep
Sleep 8+ hours nightly for tissue repair optimization.
Peptide Research & Preclinical Studies
Evidence-Based Research Findings
Study TitleType
Side Effects & Safety
Common Side Effects
• Injection site redness
• Mild fatigue
Contraindications & Warnings
• Not recommended in individuals with active malignancies due to its angiogenic effects
Long-Term Safety Data
• No adverse long-term effects reported in animal models; human data limited
🧮 Dose Calculator
Concentration
16.7
mcg/unit
Draw Volume
30
units (0.300 mL)
For a 500 mcg dose, draw 30 units on a U-100 insulin syringe
🧬
Bioavailability & Absorption
SubQ Injection
High tissue penetration and systemic distribution via subcutaneous injection
Oral Administration
Not suitable due to complete degradation in GI tract
Half-Life
2–3 hours
Degradation
Cleared by renal excretion and protease metabolism
Tissue Specificity
Tissues undergoing damage, inflammation, or requiring regenerative cues: heart, skin, brain, cornea, tendons
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Peptide Details
Molecular Weight
4963.49
Formula
C212H350N56O78S
Sequence
Ac-Ser-Asp-Lys-Pro-Thr-Phe-Ser-Pro-Lys-Glu-Lys-Lys-Cys-Asp-Glu-Leu-Ser-Lys-Leu-Tyr-Glu-Asp-Gly-Asp-Tyr-Phe-Ser-Asp-Cys-Phe-Leu-Cys-His-Val
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Legal Status & Regulatory
RegionStatus
FDANot Approved
EUNot Approved
AustraliaNot Approved
CanadaNot Approved
Storage Instructions
Lyophilized (Powder)
Store lyophilized powder at 2-8°C
Reconstituted (Mixed)
After reconstitution, maintain at 2-8°C and use within 28 days. Thymosin Beta-4 is relatively stable compared to other peptides.